Estimate of Callus induction and somatic embryogenesis in cyclamen
نویسندگان
چکیده
An efficient protocol for callus induction and somatic embryogenesis of Cyclamen premium Mill. Was developed. In this study we investigate difference between response of two explants type leaf and petiole and two medium salt concentrations MS and 1⁄2 MS on callus percentage and somatic embryo number. Media was non-significant on callus production but leaf explants was better than petiole and interaction between explants and media was significant. Leaf explants and MS medium produced the highest callus percentage in cyclamen. Media salt concentration and explants type were significant on somatic embryo number. In 1⁄2 MS medium and also in leaf explants the most somatic embryo was obtained. Also interaction between medium and explants was significant and leaf explants produced more somatic embryo in 1⁄2 MS medium but petiole explants with MS medium shown highest somatic embryo number. We found for cyclamen callus production leaf explants in MS medium is the best and for highest somatic embryo number in cyclamen Leaf explants and 1⁄2 MS is the best.
منابع مشابه
Induction of Callus and Somatic Embryogenesis from Cotyledon Explants of Fagonia indica Burm.
To explore the potential for in vitro rapid regeneration of Fagonia indica Burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on MS medium which supplemented with 1.0-5.0 µM 2,4-D. Callus was initiated from cotyledon explant on MS medium supplemented in all concentrations of 2,4-D investigated. 2,4-D was more effective than IAA and NAA for cal...
متن کاملSomatic Embryogenesis and Plant Regeneration in Evening Primrose (Oenothera biennis L.)
Callus induction responses and regeneration through callus-mediated SE were studied from evening primrose (Oenothera biennis L.) as an important medicinal plant of Onagraceae mainly known for its gamma-linoleic acid (GLA) content. The effects of cytokinins, 6-Benzylaminopurine (BAP) and N-(2-furfurylamino)1-H-purine-6-amine [Kinetin (Kin)] and their concentrations (0, 0.5, 1.0 and 1.5 mg/l) in ...
متن کاملStudy of Factors Influencing Somatic Embryogenesis in Rice (Oryza Sativa L.)
Objective: n present study effect of various factors including sucrose, agar, PEG, AgNO3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties Dom siah and Nemat. Methods: 330 explant (Mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction Murashige and Skoog (MS) medium (Murashige and Sk...
متن کاملStudy of Factors Influencing Somatic Embryogenesis in Rice (Oryza Sativa L.)
Objective: n present study effect of various factors including sucrose, agar, PEG, AgNO3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties Dom siah and Nemat. Methods: 330 explant (Mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction Murashige and Skoog (MS) medium (Murashige and Sk...
متن کاملTwo SERK genes are markers of pluripotency in Cyclamen persicum Mill.
The genetic basis of stem cell specification in somatic embryogenesis and organogenesis is still obscure. SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) genes are involved in embryogenesis and organogenesis in numerous species. In vitro culture of Cyclamen persicum immature ovules provides a system for investigating stem cell formation and maintenance, because lines forming either organs or ...
متن کامل